3 investigative approaches to advance bone tissue engineering
liver stem cells methods and protocols
... the capacity to differentiate into parenchymal hepatocytes or into bile ductular cells These stem cells may be activated to proliferate after severe liver injury or exposure to hepatocarcinogens ... University of Tokyo, Tokyo, Japan ix x Contributors YASUMASA KURODA Department of Stem Cell Biology and Histology, Tohoku University Graduate School of Medicine, Sendai, Japan JOSE MARIA MATO CIC ... able to provide metabolic functions, detoxify toxins, and synthesize proteins in place of the injured liver would be extremely useful in acute liver failure to bridge patients to recovery or to...
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Báo cáo khoa học: Hypoxia-driven proliferation of embryonic neural stem ⁄ progenitor cells – role of hypoxia-inducible transcription factor-1a ppt
... well understood Hypoxia-inducible factor (HIF)-1 is one of the key transcription factors in the response to hypoxia; it mediates a variety of adaptive cellular and systemic responses to hypoxia ... constructed into the recombinant adenoviral vector The recombinant adenoviral vector, digested with PacI to linearize the plasmid and ethanol-precipitated, was used for transfection into HEK293 cells ... neurospheres (D, E) Flow cytometric analysis showed that hypoxia, especially 10% O2, led to more NPCs in the S phase and G2 ⁄ M phase of the cell cycle (F) Cartogram of flow cytometric analysis Hypoxia...
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báo cáo hóa học:" Circulating endothelial progenitor cells: a new approach to anti-aging medicine?" docx
... endothelium to respond to physiological cues can alter mental [2], sexual [3], visual [4], and respiratory [5] ability Specifically, minute alterations in the ability of endothelium to respond to neurotransmitter ... found in the bone marrow [73,74] suggests that exhaustion in response to long-term demand may be occurring If the reparatory demands of the injury indeed lead to depletion of EPC progenitors, then ... function Circ Res 2002, 90:E89-93 Ceradini DJ, Gurtner GC: Homing to hypoxia: HIF-1 as a mediator of progenitor cell recruitment to injured tissue Trends Cardiovasc Med 2005, 15:57-63 Schomig K, Busch...
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Báo cáo hóa học: " Nutraceutical augmentation of circulating endothelial progenitor cells and hematopoietic stem cells in human subjects" potx
... of peripheral blood hematopoietic stem cell numbers was believed to be limited to postbone marrow injury conditions [43], subsequent studies have expanded this finding to situations of inflammation ... progenitor cells in fracture healing and distraction osteogenesis Bone 2008, 42(5):932-41 13 Matsumoto T, Mifune Y, Kawamoto A, et al: Fracture induced mobilization and incorporation of bone marrow-derived ... media or (b) an optimized hematopoietic growth factor cocktail as described in Materials and Methods Figure Stem-Kine Supplementation Increases Hematopoietic Progenitor Cells in Circulation PBMC...
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Báo cáo sinh học: "Safety and feasibility of third-party multipotent adult progenitor cells for immunomodulation therapy after liver transplantation–a phase I study (MISOT-I)" doc
... immunomonitoring will be performed on days 1, 3, 10, and 30, including mixed lymphocyte reactions to evaluate anti-donor reactivity, flow cytometry to describe the recipients’ leucocyte repertoire, ... macroembolism To monitor for potential embolus formation, we have specified diagnostic procedures to examine the liver and lung after intraportal and intravenous injection, respectively Toxicity related to ... Adherent, non-hematopoietic bone marrow stem cells, including MAPCs and MSCs, have been shown to beneficially modulate the anti-donor immune response in organ transplantation and to promote tissue regeneration...
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báo cáo hóa học:" Safety and feasibility of third-party multipotent adult progenitor cells for immunomodulation therapy after liver transplantation–a phase I study (MISOT-I)" pptx
... immunomonitoring will be performed on days 1, 3, 10, and 30, including mixed lymphocyte reactions to evaluate anti-donor reactivity, flow cytometry to describe the recipients’ leucocyte repertoire, ... macroembolism To monitor for potential embolus formation, we have specified diagnostic procedures to examine the liver and lung after intraportal and intravenous injection, respectively Toxicity related to ... Adherent, non-hematopoietic bone marrow stem cells, including MAPCs and MSCs, have been shown to beneficially modulate the anti-donor immune response in organ transplantation and to promote tissue regeneration...
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Transcoronary Transplantation of Progenitor Cells after Myocardial Infarction ppt
... interest relevant to this article was reported We are indebted to the staff of our catheterization laboratories; to Beate Mantz, Isabel Geweyer, and Heike Braun (study nurses); to Tina Rasper (biologic ... the day -to- day editorial activities of the Journal but is expected in addition to have his or her own independent projects Please send curriculum vitae and research interests to the Editor-in-Chief, ... inflammation Triggers leading to atherosclerosis: Risk factors for CAD Local hemodynamic factors (shear stress) Triggers probably leading to focal inflammation: Local hemodynamic factors Local vascular-remodeling...
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Báo cáo sinh học: " CNS progenitor cells and oligodendrocytes are targets of chemotherapeutic agents in vitro and in vivo" pdf
... astrocytomas to chemotherapy Cancer Res 2000, 60:4812-4818 Moore-Maxwell CA, Datto MB, Hulette CM: Chemotherapyinduced toxic leukoencephalopathy causes a wide range of symptoms: a series of four autopsies ... growth factor signal transduction J Neurosci 1990, 10:184-193 66 Tomkins CE, Edwards SN, Tolkovsky AM: Apoptosis is induced in post-mitotic rat sympathetic neurons by arabinosides and topoisomerase ... ML, Toda H, Palmer TD: Inflammatory blockade restores adult hippocampal neurogenesis Science 2003, 302:1760-1765 84 Nagahiro S, Yamamoto YL, Diksic M, Mitsuka S, Sugimoto S, Feindel W: Neurotoxicity...
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Báo cáo y học: "Identification of subpopulations with characteristics of mesenchymal progenitor cells from human osteoarthritic cartilage using triple staining for cell surface markers" docx
... progenitor cells identified in OA cartilage have marked similarities to bone marrow MPCs However, as a total population, unlike bone marrow MPCs, cartilage derived cells could not form bone in ... early to late developmental stages This knowledge may provide a basis to improve current approaches of cell based therapies for cartilage defects and to develop new strategies for guided tissue ... histological evaluation, sections were deparaffinized and either stained with haematoxylin or Alcian blue at pH 2.5, with additional Kernechtrot counterstaining, according to standard protocols...
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Báo cáo y học: "It’s all in the blood: circulating endothelial progenitor cells link synovial vascularity with cardiovascular mortality in rheumatoid arthritis" docx
... colony-stimulating factors such as granulocyte–macrophage colony-stimulating factor (GM-CSF) In rheumatoid arthritis (RA), these cells appear to traffic to RA synovium at an enhanced rate, incorporating into blood ... into newly formed vessels in an in vivo model of limb ischaemia [2] Pluripotent stem cells (PSC) in the bone marrow give rise to haemangioblasts (HA), with the potential to differentiate into ... likely to result in hypoxia, in response to which the ischaemic myocardium develops collateral vessels This compensatory angiogenesis seems to be insufficient, however, resulting in the need to perform...
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Báo cáo y học: "Reduced number and impaired function of circulating progenitor cells in patients with systemic lupus erythematosus" pot
... point to a CFU) Notice the smaller size and the lower number of emerging cells when comparing patient CFU to control CFU Figure Migratory response of circulating progenitor cells (CPCs) to tumor ... apoptosis, resulting in a shorter half-life of CPCs, can result from chronic exposure to inflammatory cytokines We specifically focused on SLE patients with quiescent disease as this enabled us to ... apoptosis Whether this indicates the existence of subsets of CPCs that intrinsically differ with respect to apoptotic responsiveness or that CPCs can adapt to culture conditions remains to be...
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Báo cáo y học: "Human palatine tonsil: a new potential tissue source of multipotent mesenchymal progenitor cells" pdf
... Washington University, Washington, DC, USA), tonsils were obtained after informed consent from patients (4 to 15 years old) undergoing tonsillectomy as a result of recurrent episodes of acute tonsillitis ... epitope profile of tonsil-derived mesenchymal progenitor cells (T-MPCs) (a) Immunofluorescence analysis of cell surface epitope profiles of T-MPCs and bone marrow-derived mesenchymal progenitor ... progenitor cells (T-MPCs) tonsil-derived mesenchymal progenitor cells (T-MPCs) (a) Histology Induced bone marrow-derived mesenchymal progenitor cell (BM-MPC) cultures are shown in the top panels; induced...
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Báo cáo y học: " Lentiviral transduction of Tar Decoy and CCR5 ribozyme into CD34+ progenitor cells and derivation of HIV-1 resistant T cells and macrophages" pdf
... hematopoietic progenitor cells by using lentiviral vectors The transduced progenitor cells differentiated normally into mature thymocytes in vivo in thy/liv grafts of SCID-hu mice and into normal ... In these studies, our goal is to determine the utility of these constructs when introduced into human CD34+ progenitor cells via an HIV-1 based lentiviral vector to derive HIV resistant differentiated ... vector preparation was determined in 293T cells as described previously and ranged from to × 108 TU/ml Multiple aliquots were made and stored at -70°C Isolation of CD34+ hematopoietic progenitor...
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Báo cáo y học: "Prenatal exposure of ethanol induces increased glutamatergic neuronal differentiation of neural progenitor cells" pot
... between excitatory and inhibitory synapses is related to autistic symptoms [23] This imbalance of excitation and inhibition could be due to the increased excitatory signaling, or to a reduction ... reduction in inhibition due to a reduction in inhibitory signaling [24] Increasing the numerical or functional balance of excitatory vs inhibitory cells can lead to a hyper-excitable state, which ... sufficient to initiate the development of neuronal lineages and to promote the generation of progenitor cells that have a capacity to differentiate Importantly, proneural genes have been shown to have...
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Báo cáo y học: " Enhanced self-renewal of hematopoietic stem/progenitor cells mediated by the stem cell gene Sall4" doc
... early primitive cells We next extended our study to a myeloid progenitor cell line (32D), to determine how Sall4 may affect hematopoietic progenitor cell differentiation 32D cells were cultured ... cloned into the SalI/NotI site of entry vector pENTR3C (Invitrogen, Carlsbad, CA) A Gateway LR reaction was carried out to subclone the cDNA into the lentiviral mammalian expression vector pDEST-CMVFG12 ... similar results were obtained To date, only several factors have been reported to expand HSC/HPCs in vitro while their stem cell characteristics were not impaired To test whether Sall4expanded...
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báo cáo khoa học: "Optical characterization of colloidal CdSe quantum dots in endothelial progenitor cells" pptx
... and highest = 100%) To ensure the intracellular localization of QDs, a series of 163 sequential images that covers the whole cell volume (from the top down to the bottom, total thickness 19.6 ... to the excitation wavelength can be theoretically expected when the excitation energy is not too high compared with the excitonic energy of QDs (i.e., in the range of one-photon and multiphoton ... progenitor cells Arterioscler Thromb Vasc Biol 2008, 28(9):1584-1595 Fadini GP, Baesso I, Albiero M, Sartore S, Agostini C, Avogaro A: Technical notes on endothelial progenitor cells: ways to escape...
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Báo cáo y học: " Profibrotic potential of Prominin-1+ epithelial progenitor cells in pulmonary fibrosis" docx
... cells [9-11] Furthermore, bone marrow-derived stem and progenitor cells isolated from the lung [11,12] or from other tissues [13-15] have the capacity to differentiate into pulmonary epithelial ... complementary to transcripts of the analyzed genes (Additional file 1) Histology, immunocytochemistry and phagocytosis assay Formalin-fixed, paraffin-embedded lung sections were stained with hematoxylin ... prominin-1-expressing progenitors Discussion We recently identified a population of bone marrowderived lung resident prominin-1+ epithelial progenitor cells with the capacity to differentiate into alveolar type...
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Báo cáo y học: " Peripheral infusion of rat bone marrow derived endothelial progenitor cells leads to homing in acute lung injury" pps
... Apoptosis seems to be a major pathway to EC death in ALI, and by the use of a caspase inhibitor to block the execution of apoptosis, there is some indirect evidence that influencing such apoptosis ... of progenitor cells Furthermore, these cells might be crucial to repair the lung in order to maintain the organ structure, as they integrate in the tissue and seem to differentiate or to fuse with ... (Becton Dickinson) for 10 at room temperature After washing twice with ml PBS, cells were measured in a BD LSR flow cytometer (Becton Dickinson) using Cell Quest software (Becton Dickinson) To...
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Báo cáo y học: "Sequestration and homing of bone marrow-derived lineage negative progenitor cells in the lung during pneumococcal pneumonia" ppsx
... (Figure 1C) Although QDs may be cytotoxic to live cells due to their reactive metal core [23], we used low QD concentrations that have been shown to be noncytotoxic and have no impact on cell ... these progenitor cells also remained in the lung tissues and rarely migrated into the alveolar air spaces, suggesting that BM-derived progenitor cells sequester and home to lung tissues Several ... inflammatory response induced by pneumonia is also characterized by an increase in circulating proinflammatory mediators [8-10], of which several (such as G-CSF and GM-CSF) are known to release hematopoietic...
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Báo cáo y học: "Relative percentage and zonal distribution of mesenchymal progenitor cells in human osteoarthritic and normal cartilage" potx
... cartilage repair Eur J Radiol 2006, 57:3-8 33 Wakitani S, Imoto K, Yamamoto T, Saito M, Murata N, Yoneda M: Human autologous culture expanded bone marrow mesenchymal cell transplantation for repair ... growth [10,11] or differential adhesion to fibronectin [12] to the differential uptake of cell-penetrating dyes [13] or their ability to grow out of cartilage tissue [9] Alternatively, the expression ... cartilage from healthy donors, all normal-appearing tissue was harvested To standardize the procedure and to avoid contamination of the chondrocytes with bone marrow cells, the subchondral lamella was...
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